Response of Microbial Community to Petroleum Stress and Phosphate Dosage in Sediments of Jiaozhou Bay,China     

《青岛海洋大学学报(英文版)》2014,(2):249-256

The dynamic microcosms were used to evaluate the effect of oil spills on microbial ecological system in marine sedi- ment and the enhancement of nutrient on the oil removal. The function and structure of microbial community caused by the oil pollu-tion and phosphate dosage were simultaneously monitored by dehydrogenase activity assay and PCR-denaturing gradient gel elec- trophoresis （DGGE） techniques. The results indicated that the amount of total bacteria in all dynamic microcosms declined rapidly with incubation time. The number of petroleum-degrading bacteria and the activity of sediment dehydrogenase were gradually en-hanced by petroleum in the oil-treated microcosms, while they both showed no obvious response to phosphate dosage. In comparison, phosphate spiked heterotrophic bacteria and they showed a significant increase in amount. DGGE profiles indicated that petroleum dosage greatly changed community structure, and the bacteria belonged to class Deltaproteobacteria, and phyla Bacteroidetes and Chlorobi were enriched. This study demonstrated that petroleum input greatly impacted the microbial community structure and con- sequently the marine sediment petroleum-degrading activity was enhanced. Phosphate dosage would multiply heterotrophic bacteria but not significantly enhance the petroleum degradation.  相似文献   

Temporal Change of Runoff and Sediment Load and their Differential Response to Human Activities：A Case Study for a Well-vegetated Mountain Watershed of Southern China   总被引：1，自引：1，他引：0  

ZHENG Ming-guo  SUN Li-ying  YAN Ming 《山地科学学报》2014,11(1):73-85

This study examined the temporal trends of runoff and sediment load and their differential response to human activities in the Lishui river,a tributary of the Yangtze river in southern China.The long-term observation data at four gauging stations,generally involving two periods from 1954 to 1985 and from 2007 to 2011,were used.We detected no significant temporal trend for both the annual runoff volume（Q） and the annual suspended Sediment Load（SL） over more than 30 years before 1985.The flow duration curves and the Suspended Sediment Concentration（SSC） also hold constant before 1985.Compared with the period before 1985,SL has decreased by about 80% though Q remains unchanged for the period after 2007.Detailed examination shows that the flow duration curves after 2007 have changed with a significant decrease in the high-flow component,which acts as a major cause for the decreasing SL.In addition,SSC has decreased by several times,which also contributes to the decrease in SL after 2007.Both decreases in high-flow discharges and in SSC can be linked with recent human activities,mainly including vegetation establishment and dam constructions.The constant Q and the decreasing SL are also reported for the main stream of the Yangtze River and other major rivers in southern China,although they are orders of magnitude larger than our study area in drainage area size.The present study highlights the importance of high-flow discharges on SL and suggests that the use of SL is more appropriate to reflect environmental change than Q.  相似文献   

春季长江口及其邻近海域溶解有机物的分布、组成及降解行为研究     

陈博洋  陈容  陈岩  杨桂朋 《海洋科学》2022,46(11):1-14

2021年3月对长江口及其邻近海域9个断面溶解氨基酸(THAA)、溶解有机碳(DOC)和溶解有机氮(DON)的分布和组成进行了调查研究。结果表明:THAA的浓度范围为1.24~4.71μmol/L,平均浓度为(2.61±0.85)μmol/L;DOC的浓度范围为118.17~450.58μmol/L,平均浓度为(149.73±33.34)μmol/L;DON的浓度范围为10.48~24.45μmol/L,平均浓度为(13.80±1.81)μmol/L。THAA、DOC和DON的水平分布表现出近岸高于远岸的特点,表明陆源输入对调查区域的THAA等分布有重要影响。而THAA与D-天门冬氨酸、D-谷氨酸、D-丝氨酸和D-丙氨酸的D/L比均呈显著负相关,与天门冬氨酸/β-丙氨酸(Asp/β-Ala)呈正相关,表明细菌活动是影响THAA浓度变化的重要因素。长江口及邻近海域的优势氨基酸为L型氨基酸,D型氨基酸和非蛋白型氨基酸的占比仅为7.34%。基于C/N比值分析,长江口及其邻近海域溶解有机物(DOM)的来源受到长江冲淡水和台湾暖流的共同影响。THAA-C%、THAA-N%、降解因子和反应活性指数表明:水平方向上盐度接近于0的淡水区域和盐度高于34的远海区域降解程度较高;垂直方向上随着深度的增加,有机物的降解程度逐渐升高。调查区域有机物的降解程度主要受到陆源输入和细菌活动的影响。  相似文献   

黏土中防沉板—桩复合基础地震反应特性研究     

关盛杰  孔德森  王晓敏  巩越 《海洋工程》2022,40(1):116-124

为了研究海底防沉板—桩复合基础在地震荷载作用下的动力反应特性,以我国南海深水工程实例为研究对象,利用Flac3D有限差分仿真软件建立了计算模型,土体采用Mohr-Coulomb本构模型,模型底部输入EL Centro地震波,对不同桩长的复合基础进行分析计算.在该特定工程背景下,研究结果表明:随着桩长的增加,防沉板顶部加速度放大系数呈减小趋势;地震荷载下,复合基础发生震陷,沉降量在地震波加速度峰值过后趋于稳定;当桩长为6m时,复合基础的水平振动程度和震陷量最小;由于桩基础把震动能量传输到深部土层中,复合基础周围土体的加速度响应值小于远场土体.在动力时域内,防沉板与桩连接处弯矩最大,需要在此处增设加固装置.  相似文献   

海上风电基础下放通过浪溅区响应特性研究     

张浦阳  李彦娥  丁红岩  乐丛欢 《海洋工程》2022,40(4):77-87

多筒型导管架基础下放安装过程筒顶开口,筒内气—水自由置换,浪溅区下放过程由于浮力、冲击载荷、附加质量、阻尼效应等因素影响具有很强的非线性。开展模型试验研究波浪荷载下外界控制因素对四筒导管架基础下放过程气垫响应、基础运动和吊缆张力响应的影响机理,探究气垫结构对基础下放过程附加质量和固有周期的影响。结果表明：初始下放过程,筒内气体积聚,气垫压力非线性增长,随着下放深度增大,气垫压力趋于稳定;气垫压力响应趋势与基础下放速度和开孔率相关,波浪荷载仅影响气垫响应幅值;筒型基础下放穿越浪溅区阶段受到砰击荷载作用,气垫结构对砰击荷载产生缓冲效果;考虑气垫—水柱耦合作用时,顶盖即将入水阶段,气弹簧和水弹簧耦合作用下整体刚度增大,基础固有周期出现减小趋势。  相似文献   

循环载荷作用下损伤船体梁极限强度研究     

吴剑国  邵智华  申屠晨楠  王锦琪  叶帆  傅何琪  王福花 《海洋工程》2022,40(5):89-97

为了研究循环载荷作用下扶强材初始损伤对其极限强度的影响,进行了14组扶强材的循环加载试验和分析。构造了考虑材料累积损伤完整、断筋和大变形的扶强材单元极限承载力计算公式,提出了相应循环载荷作用下损伤扶强材单元的端缩曲线表达式和船体梁极限强度计算的简化逐步破坏法。编制了循环载荷作用下船体梁损伤极限强度计算程序,进行了船体梁极限强度计算,并与有限元结果进行对比。研究结果表明：改进的损伤扶强材模型可较为准确地描述扶强材材料损伤的完整、断筋和大变形的极限承载力退化情况,扶强材腹板断裂的损伤相较初始大变形及材料累积损伤形式承载力下降程度更明显;所提出的循环载荷作用下损伤船体梁极限强度计算的简化逐步迭代方法,能定量地计算扶强材在不同类型损伤下的极限承载力退化程度,具有较高精度,方便易行,可应用于工程设计。  相似文献   

单体喷水推进船舶动力定位控制方法研究     

李刚强  谌栋梁  葛卓  周冠泽  熊文 《海洋工程》2022,40(5):78-88

面向一型喷水推进器为动力定位执行机构的单体喷水推进船舶,开展了船舶仿真模型建立、推力分配及进一步动力定位相关控制算法设计与仿真模拟。针对传统的定步长Kalman滤波提出了改进方案,同时考虑到单体喷水推进船舶的特殊性,对推力分配算法进行了优化,并基于荷载前馈的PD控制算法开展了不同工况下的仿真试验。试验结果表明,所提出的喷水推进推力分配算法能够有效控制一型单体喷水推进船舶实现定点、定艏向、移位、转艏等动力定位典型功能,并具备一定抗外部干扰能力。  相似文献   

一种基于RGB特征提取的电子地图载负量计算方法     

江南  张昊  曹亚妮  颜玉龙 《测绘工程》2013,(6):39-42,46

电子地图以灵活多变的显示形式,可以在不同比例尺下显示与地理环境有关的各种信息,且在一定限度内可进行无级显示.然而,在电子地图缩放过程中如果不对屏幕载负量加以控制,往往容易造成各要素互相挤压,但如果只是对屏幕载负量简单限制又满足不了用户多尺度的需求.因此,为了提高地图信息传输的有效性及可控性,文中提出基于RGB特征提取电子地图载负量的新的计算方法,并进行相关实验分析.  相似文献   

Analyses of suspended sediment loads in Slovenian rivers     

Nejc Bezak  Mojca Šraj  Matjaž Mikoš 《水文科学杂志》2013,58(6):1094-1108

ABSTRACT

Suspended solids are present in every river, but high quantities can worsen the ecological conditions of streams; therefore, effective monitoring and analysis of this hydrological variable are necessary. Frequency, seasonality, inter-correlation, extreme events, trends and lag analyses were carried out for peaks of suspended sediment concentration (SSC) and discharge (Q) data from Slovenian streams using officially monitored data from 1955 to 2006 that were made available by the Slovenian Environment Agency. In total more than 500 station-years of daily Q and SSC data were used. No uniform (positive or negative) trend was found in the SSC series; however, all the statistically significant trends were decreasing. No generalization is possible for the best fit distribution function. A seasonality analysis showed that most of the SSC peaks occurred in the summer (short-term intense convective precipitation produced by thunderstorms) and in the autumn (prolonged frontal precipitation). Correlations between Q and SSC values were generally relatively small (Pearson correlation coefficient values from 0.05 to 0.59), which means that the often applied Q–SSC curves should be used with caution when estimating annual suspended sediment loads. On average, flood peak Q occurred after the corresponding SSC peak (clockwise-positive hysteresis loops), but the average lag time was rather small (less than 1 day).